CCAR1 Antibody

ProSci
Product Code: PSI-42-273
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-273-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
42-273 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-273 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CCAR1, cell division cycle and apoptosis regulator 1, CARP-1, CARP1, MGC44628, RP11-437A18.1, OTTHUMP00000019709, cell-cycle and apoptosis regulatory protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 120kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 132kDa according to Mouse NP_080477.1 and Rat NP_001102005.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Kim JH, Yang CK, Heo K, Roeder RG, An W, Stallcup MR, CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes. Molecular cell 2008 Aug 31 (4): 510-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: RYCKERPSKDKE
NCBI Gene ID #:
55749
NCBI Official Name:
cell division cycle and apoptosis regulator 1
NCBI Official Symbol:
CCAR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060707.2
Protein GI Number:
46852388
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis
SPECIFICITY:
.
Swissprot #:
Q8IX12