CDKN2A Antibody

ProSci
Product Code: PSI-45-033
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-033-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-033 (1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
2 / 2

45-033 (1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
CDKN2A, p16INK4a, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) , HGNC:1787, ARF, CDK4I, CDKN2, CMM2, INK4, INK4a, MLM, MTS1, TP16, p14, p14ARF, p16, p16INK4, p19 , CDK4 inhibitor p16-INK4, cell cycle negative regulator beta, cyclin-
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Background References:
  • Mawrin C, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk Alterations of cell cycle regulators in gliomatosis cerebri. J Neurooncol. 2005 Apr;72(2):115-22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HARIDAAEGPSDIPD
NCBI Gene ID #:
1029
NCBI Official Name:
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol:
CDKN2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000068.1, NP_001182061.1
Protein GI Number:
4502749
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Signal Transduction ,Cancer
SPECIFICITY:
This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).
Swissprot #:
P42771