CEP290 Antibody

ProSci
Product Code: PSI-42-225
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-225-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-225 (2.5ug/ml overnight staining of cell lines OPCT-1 (A) and MDA468 (fB) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue). Data kindly provided by Dr S, McArdle, and obtained by Sumanjeet Malhi , John van Geest Research Centr

42-225 (2.5ug/ml overnight staining of cell lines OPCT-1 (A) and MDA468 (fB) with Alexa Fluor 568 (red) and nuclear counter staining with DAPI (blue). Data kindly provided by Dr S, McArdle, and obtained by Sumanjeet Malhi , John van Geest Research Centr

Further Information

Additional Names:
CEP290, centrosomal protein 290kDa, 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16, CTCL tumor antigen se2-2, monoclonal antibody 3H11 antigen, nephrocytsin-6, prostate cancer antigen T21
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunocytochemistry: Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining. Recommended concentration: 2-3ug/ml
Background References:
  • Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A. Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice. J. Clin. Invest. 2008 May 118 (5): 1955-64.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-RNSKHLKQQQYRAEN
NCBI Gene ID #:
80184
NCBI Official Name:
centrosomal protein 290kDa
NCBI Official Symbol:
CEP290
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079390.3
Protein GI Number:
109255234
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290.
Swissprot #:
O15078