CHMP5 Antibody

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ProSci
Product Code: PSI-45-399
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-399-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-399 (xug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-399 (xug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CHMP5, chromatin modifying protein 5 , HGNC:26942, C9orf83, CGI-34, HSPC177, PNAS-2, SNF7DC2, SNF7 domain containing 2, chromosome 9 open reading frame 83, Vps60
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;280(11): 10548-55.; PMID: 15644320). Recommended concentration: 1-3ug/ml.
Background References:
  • Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-NKDGVLVDEFGLPQ
NCBI Gene ID #:
51510
NCBI Official Name:
charged multivesicular body protein 5
NCBI Official Symbol:
CHMP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057494.3
Protein GI Number:
20127558
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9NZZ3