cofilin Antibody

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ProSci
Product Code: PSI-79-170
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-170-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from COLO205 cells using cofilin (Phospho-Ser3).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using cofilin (Phospho-Ser3).
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Western blot analysis of lysed extracts from COLO205 cells using cofilin (Phospho-Ser3).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using cofilin (Phospho-Ser3).

Documents

Further Information

Additional Names:
CFL, HEL-S-15, HER2, MLN19, NEU, NGL, CFL, CFL1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Background References:
  • Kobayashi M, et al. (2006) EMBO J 25 (4): 713-26. Wang Y, et al. (2005) Biol Chem 280 (13): 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
cofilin (Phospho-Ser3) antibody was raised against a peptide sequence around phosphorylation site of serine 3 (M-A-S (p) -G-V) derived from Human cofilin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1072
NCBI Official Name:
cofilin 1 (non-muscle)
NCBI Official Symbol:
CFL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
NP_005498.1
Protein GI Number:
5031635
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Swissprot #:
P23528