COL4A3BP Antibody

ProSci
Product Code: PSI-45-412
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-412-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-412 (0.5ug/ml) staining of Mouse Heart lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-412 (0.5ug/ml) staining of Mouse Heart lysate (A) + peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein , CERT, CERTL, FLJ20597, GPBP , STARD11, Goodpasture antigen-binding protein, START domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60-65kDa band observed in Mouse Heart lysates (calculated MW of 71.1kDa according to Mouse NP_075909.1). An additional band of unknown identity was also consistently observed at 25kDa. Both bands were successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1ug/ml. Peimary incubation 1 hour at room temperature.
Background References:
  • Swanton C, Marani M, Pardo O, Warne PH, Kelly G, Sahai E, Elustondo F, Chang J, Temple J, Ahmed AA, Brenton JD, Downward J, Nicke B. Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs. Cancer. 2007 Jun;11(6):498-512.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: DHDSAPLNNRC
NCBI Gene ID #:
10087
NCBI Official Name:
collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
NCBI Official Symbol:
COL4A3BP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005704.1, NP_112729.1
Protein GI Number:
5031717
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1)
Swissprot #:
Q9Y5P4