Coronin 1 Antibody

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ProSci
Product Code: PSI-45-421
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-421-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 overexpressing CORO1A and probed with 45-421 (mock transfection in first lane).
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45-421 (0.3ug/ml) staining of Human Lymphoblastoid (RIPA buffer, (35ug protein in RIPA buffer). Detected by chemiluminescence.
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HEK293 overexpressing CORO1A and probed with 45-421 (mock transfection in first lane).
45-421 (0.3ug/ml) staining of Human Lymphoblastoid (RIPA buffer, (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CORO1A, Coronin 1, TACO, p57, CLABP, HCORO1, CLIPINA, Clabp TACO, coronin, actin binding protein, 1A, coronin-1, coronin, actin-binding, 1A, coronin, actin-binding protein, 1A, FLJ41407, MGC117380,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human Lymphoblastoid and Human T cell line MOLT4 lysates (calculated MW of 51.0kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Small Intestine. Recommended concentration: 3.75ug/ml.
Background References:
  • Ferrari G, Langen H, Naito M, Pieters J. A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell. 1999 May 14;97(4):435-47.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KRLDRLEETVQAK
NCBI Gene ID #:
11151
NCBI Official Name:
coronin, actin binding protein, 1A
NCBI Official Symbol:
CORO1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009005.1
Protein GI Number:
5902134
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P31146