CPT1B Antibody

ProSci
Product Code: PSI-45-427
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-427-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-427 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-427 (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
CPT1B, carnitine palmitoyltransferase I, muscle, carnitine palmitoyltransferase 1B (muscle) , CPT1-M, M-CPT1, KIAA1670, CPTI, muscle, carnitine O-palmitoyltransferase I, mitochondrial muscle isoform, carnitine palmitoyltransferase 1A (muscle), carnitine palmitoyltransferase 1B, carnitine palmitoyltransferase 1B (muscle), CPT1M, FLJ55729, FLJ58750, MCCPT1, MCPT1, carnitine palmitoyltransferase I-like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5ug/ml.
Background References:
  • Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. Biochim Biophys Acta. 1996 Jun 7;1307(2):157-61.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DIADLFQVPKAYS
NCBI Gene ID #:
1375
NCBI Official Name:
carnitine palmitoyltransferase 1B (muscle)
NCBI Official Symbol:
CPT1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_689452.1, NP_689451.1, NP_004368.1, NP_001138609.1, NP_001138607.1, NP_001138606.1, NP_001138608.1
Protein GI Number:
4758050
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise human isoforms a (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).
Swissprot #:
Q92523