ProSci

D53 Antibody

Product Code:
 
PSI-45-454
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-454 (0.1ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-454 (0.1ug/ml) staining of Human Breast cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-454-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
TPD52L1, hD53, tumor protein D52-like 1, D53, MGC8556, TPD52L2, tumor protein D52-like 2, tumor protein D53
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band observed in Human Breast cancer lysates (calculated MW of 22.5kDa according to NP_003278.1). Recommended concentration: 0.1-0.3ug/ml. See also our D52 and D54 products.
Background References:
  • Boutros R, Bailey AM, Wilson SH, Byrne JA. Alternative splicing as a mechanism for regulating 14-3-3 binding: interactions between hD53 (TPD52L1) and 14-3-3 proteins. J Mol Biol. 2003 Sep 19;332(3):675-87.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EPLQGTDEDAVASAD
NCBI Gene ID #:
7164
NCBI Official Name:
tumor protein D52-like 1
NCBI Official Symbol:
TPD52L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003278.1, NP_001003395.1 , NP_001003396.1, NP_001003397.1
Protein GI Number:
4507641
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoforms 1, 3 and 4 (NP_003278.1, NP_001003396.1 and NP_001003397.1 resp.).
Swissprot #:
Q16890