ProSci

DIAPH1 Antibody

Product Code:
 
PSI-42-285
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
42-285 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-285 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-42-285-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DIAPH1, diaphanous homolog 1 (Drosophila), DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1, diaphanous 1, diaphanous-1, diaphanous-related formin 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141kDa according to Human NP_005210.3 and 139kDa according to Mouse NP_031884.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Higashi T, Ikeda T, Shirakawa R, Kondo H, Kawato M, Horiguchi M, Okuda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets J Biol Chem. 2008 Mar 28;283(13):8746-55
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-QTKTSKAKKDQ
NCBI Gene ID #:
1729
NCBI Official Name:
diaphanous-related formin 1
NCBI Official Symbol:
DIAPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005210.3, NP_001073280.1
Protein GI Number:
119395758
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Swissprot #:
O60610