ProSci

Dispatched homolog 1 Antibody

Product Code:
 
PSI-45-482
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-482 (2.5ug/ml) staining of U87. Detected by immunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.

45-482 (2.5ug/ml) staining of U87. Detected by immunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.

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PSI-45-482-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DISP1, DISPA, MGC13130, MGC16796, DKFZP434I0428, dispatched A, DISP1, dispatched homolog 1, dispatched homolog 1 (Drosophila), FLJ43740, MGC104180
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1ug/ml.Immunofluorescence: Cell membrane staining in cell line U87. These data corresponds to earlier findings in literature with different antibodies (Etheridge et al, Development. 2010 Jan;137(1):133-40; PMID: 20023168).
Background References:
  • Burke R, Nellen D, Bellotto M, Hafen E, Senti KA, Dickson BJ, Basler K. Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. Cell. 1999 Dec 23;99(7):803-15.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-SELSGESLLIKTL
NCBI Gene ID #:
84976
NCBI Official Name:
dispatched homolog 1 (Drosophila)
NCBI Official Symbol:
DISP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116279.2
Protein GI Number:
25952134
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q96F81