ProSci

DR6 (Luke-1) Monoclonal Antibody

Product Code:
 
PSI-XA-1015
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rat
Antibody Isotype:
 
Rat IgG2a
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Flow Cytometry
Storage:
 
DR6 antibody can be stored at 4˚C, stable for one year.
 

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-XA-1015-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Note:
DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
Background References:
  • Identification and functional characterization of DR6, a novel death domain-containing TNF receptor : G. Pan, et al.; FEBS Lett 431, 351 (1998).
  • Impaired c-Jun amino terminal kinase activity and T cell differentiation in death receptor 6-deficient mice : H. Zhao, et al.; J. Exp. Med. 194, 1441 (2001).
  • Enhanced CD4+ T cell proliferation and Th2 cytokine production in DR6-deficient mice:J. Liu, et al.; Immunity 15, 23 (2001).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335).
NCBI Gene ID #:
27242
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 21
NCBI Official Symbol:
TNFRSF21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
DR6 (monoclonal Luke-1) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Apoptosis
Swissprot #:
O75509
User NOte:
Optimal dilutions for each application to be determined by the researcher.