Elk1 Antibody

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ProSci
Product Code: PSI-79-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-283-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Ab-389).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Elk1 (Ab-389).
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Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Ab-389).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Elk1 (Ab-389).

Documents

Further Information

Additional Names:
VIL2, ELK1, ETS-domain protein Elk-1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
Background References:
  • Janknecht R, et al. (1993) EMBO J. 12 (13): 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Elk1 (Ab-389) antibody was raised against a peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1.
NCBI Gene ID #:
2002
NCBI Official Name:
ELK1, member of ETS oncogene family
NCBI Official Symbol:
ELK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
NP_001107595.1
Protein GI Number:
166362732
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Elk1 protein.
Swissprot #:
P19419