EXO1 Antibody

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ProSci
Product Code: PSI-45-556
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-556-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-556 (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-556 (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Further Information

Additional Names:
EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5ug/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Kim JC, Roh SA, Yoon YS, Kim HC, Park IJ. MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria. Cancer Genet Cytogenet. 2007 Jul 15;176(2):172-4. No abstract available.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HRNYSPRPESGT
NCBI Gene ID #:
9156
NCBI Official Name:
exonuclease 1
NCBI Official Symbol:
EXO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003677.3, NP_006018.3, NP_569082.1
Protein GI Number:
39995069
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1)
Swissprot #:
Q9UQ84