EYA1 Antibody

ProSci
Product Code: PSI-42-258
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-258-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-258 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-258 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
EYA1, eyes absent homolog 1 (Drosophila), BOP, BOR, MGC141875, Eyes absent, Drosophila, homolog of, 1, Melnick-Fraser syndrome, OTTHUMP00000195053, eyes absent 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in lysates of cell line HEK293 (calculated MW of 60.7kDa according to NP_742056.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Orten DJ, Fischer SM, Sorensen JL, Radhakrishna U, Cremers CW, Marres HA, Van Camp G, Welch KO, Smith RJ, Kimberling WJ. Branchio-oto-renal syndrome (BOR): novel mutations in the EYA1 gene, and a review of the mutational genetics of BOR. Human Mutation 2008 Apr 29 (4): 537-44.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TDPTAEYSTIHSP
NCBI Gene ID #:
2138
NCBI Official Name:
eyes absent homolog 1 (Drosophila)
NCBI Official Symbol:
EYA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_742057.1, NP_000494.2, NP_742056.1
Protein GI Number:
26667222
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all isoforms (NP_742057.1; NP_000494.2; NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1
Swissprot #:
Q99502