FACL4 Antibody
| Code | Size | Price |
|---|
| PSI-45-563-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ACSL4, acyl-CoA synthetase long-chain family member 4, FACL4, fatty-acid-Coenzyme A ligase, long-chain 4, ACS4, LACS 4, lignoceroyl-CoA synthase, Fatty acid coenzyme A ligase, long-chain 4, MRX63, LACS4, MRX68, acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line HepG2 (calculated MW of 79.2kDa according to NP_075266.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine and Brain (Cortex). Recommended concentration: 3.75ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Piccini M, Vitelli F, Bruttini M, Pober BR, Jonsson JJ, Villanova M, Zollo M, Borsani G, Ballabio A, Renieri A. FACL4, a new gene encoding long-chain acyl-CoA synthetase 4, is deleted in a family with Alport syndrome, elliptocytosis, and mental retardation. Genomics. 1998 Feb 1;47(3):350-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HYLKDIERMYGGK
NCBI Gene ID #:
2182
NCBI Official Name:
acyl-CoA synthetase long-chain family member 4
NCBI Official Symbol:
ACSL4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004449.1, NP_075266.1
Protein GI Number:
4758332
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
Swissprot #:
O60488