G6PD Antibody
| Code | Size | Price |
|---|
| PSI-45-644-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Guindo A, Fairhurst RM, Doumbo OK, Wellems TE, Diallo DA. X-Linked G6PD Deficiency Protects Hemizygous Males but Not Heterozygous Females against Severe Malaria. PLoS Med. 2007 Mar 13;4(3):e66
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-STNSDDVRDEKVK
NCBI Gene ID #:
2539
NCBI Official Name:
glucose-6-phosphate dehydrogenase
NCBI Official Symbol:
G6PD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000393.4 , NP_001035810.1
Protein GI Number:
109389365
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
Swissprot #:
P11413