GAL Antibody

ProSci
Product Code: PSI-42-216
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-216-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-216 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-216 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
GAL, galanin prepropeptide, GALN, GLNN, GMAP, MGC40167, galanin, galanin-message-associated peptide, galanin-related peptide
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 13.3kDa according to Human NP_057057.2 and of 13.5kDa according to Mouse NP_034383.1 and Rat NP_150240.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration: 0.2-0.5ug/ml.
Background References:
  • Sugimoto T, Seki N, Shimizu S, Kikkawa N, Tsukada J, Shimada H, Sasaki K, Hanazawa T, Okamoto Y, Hata A, The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma. Genes, chromosomes & cancer 2009 Feb 48 (2): 132-42.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-HRSFSDKNGLTSK
NCBI Gene ID #:
51083
NCBI Official Name:
galanin/GMAP prepropeptide
NCBI Official Symbol:
GAL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057057.2
Protein GI Number:
27804313
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
Swissprot #:
P22466