GBX2 Antibody

ProSci
Product Code: PSI-45-659
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-659-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-659 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-659 (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
GBX2, gastrulation brain homeobox 2 , gastrulation brain homeo box 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3kDa according to NP_001476.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH-3T3 cell line for this particular batch.Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts. Recommended concentration: 4-8ug/ml. Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Background References:
  • Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD. Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QGKDESKVEDDPK
NCBI Gene ID #:
2637
NCBI Official Name:
gastrulation brain homeobox 2
NCBI Official Symbol:
GBX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001476.2
Protein GI Number:
45593144
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P52951