GEF5 Antibody

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ProSci
Product Code: PSI-45-664
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-664-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-664 (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-664 (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
GEF5, DKFZp686N1969, ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Background References:
  • Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1;13(3):323-34. Epub 2003 Dec 8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DKEIDQNSQQEE
NCBI Gene ID #:
7984
NCBI Official Name:
Rho guanine nucleotide exchange factor (GEF) 5
NCBI Official Symbol:
ARHGEF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005426.2
Protein GI Number:
50959191
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q12774