GERP Antibody

ProSci
Product Code: PSI-45-667
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-667-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-667 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

45-667 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Further Information

Additional Names:
TRIM8, tripartite motif-containing 8, GERP, RNF27, ring finger protein 27, tripartite motif protein TRIM8, glioblastoma expressed ring finger protein, RP11-47A8.4,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:No signal obtained yet but low background observed in human brain and human kidney lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Kidney shows preferential nuclear staining in convoluted tubules. Recommended concentration, 4-6ug/ml.
Background References:
  • Vincent SR, Kwasnicka DA, Fretier P. A novel RING finger-B box-coiled-coil protein, GERP. Biochem Biophys Res Commun. 2000 Dec 20;279(2):482-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-YGQPSTKHYVTS
NCBI Gene ID #:
81603
NCBI Official Name:
tripartite motif containing 8
NCBI Official Symbol:
TRIM8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_112174.2
Protein GI Number:
148596968
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9BZR9