GLuR5 Antibody
| Code | Size | Price |
|---|
| PSI-45-679-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3 , EEA3 , GLR5 , excitatory amino acid receptor 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24(41):8986-93.; PMID: 15483117). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Background References:
- Martin S, Nishimune A, Mellor JR, Henley JM. SUMOylation regulates kainate-receptor-mediated synaptic transmission. Nature. 2007 May 17;447(7142):321-5. Epub 2007 May 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: QCKQTHPTNSTS
NCBI Gene ID #:
2897
NCBI Official Name:
glutamate receptor, ionotropic, kainate 1
NCBI Official Symbol:
GRIK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000821.1, NP_783300.1
Protein GI Number:
4504117
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
Swissprot #:
P39086