GOLGA3 Antibody

ProSci
Product Code: PSI-45-686
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-686-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-686 (0.1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-686 (0.1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
GOLGA3, HGNC:4426, GCP170, MEA-2 , Golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi membrane associated protein, Golgi peripheral membrane protein, SY2/SY10 protein, golgin-160, golgin-165, male enhanced antigen-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Hicks SW, Machamer CE. The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information. J Biol Chem. 2002 Sep 27;277(39):35833-9. Epub 2002 Jul 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HSQSRASKEGPGE
NCBI Gene ID #:
2802
NCBI Official Name:
golgin A3
NCBI Official Symbol:
GOLGA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005886.2
Protein GI Number:
30089940
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q08378