GPSM2 Antibody

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ProSci
Product Code: PSI-45-698
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-698-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-698 (0.01ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-698 (0.01ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
GPSM2, G-protein signalling modulator 2 (AGS3-like, C. elegans) , HGNC:29501, LGN, Pins, LGN protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03ug/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Background References:
  • Yasumi M, Sakisaka T, Hoshino T, Kimura T, Sakamoto Y, Yamanaka T, Ohno S, Takai Y. Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division. J Biol Chem. 2005 Feb 25;280(8):6761-5. Epub 2005 Jan 4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-NRLKGKKYKTNSSTK
NCBI Gene ID #:
29899
NCBI Official Name:
G-protein signaling modulator 2
NCBI Official Symbol:
GPSM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_037428.2
Protein GI Number:
31543053
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P81274