GPX3 Antibody

ProSci
Product Code: PSI-45-699
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-699-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-699 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-699 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-699 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-699 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
GPX3, glutathione peroxidase 3 (plasma), GPx-P, GSHPx-3, GSHPx-P , extracellular glutathione peroxidase, plasma glutathione peroxidase 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Colon. Recommended concentration: 5ug/ml. Frozen sections of Porcine Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
Background References:
  • Chu FF, Esworthy RS, Doroshow JH, Doan K, Liu XF. Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents. Blood. 1992 Jun 15;79(12):3233-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog,
Immunogen:
The immunogen for this antibody is: C-NQFGKQEPGENSE
NCBI Gene ID #:
2878
NCBI Official Name:
glutathione peroxidase 3 (plasma)
NCBI Official Symbol:
GPX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002075.2
Protein GI Number:
6006001
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Swissprot #:
P22352