GPX7 Antibody

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ProSci
Product Code: PSI-45-702
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-702-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-702 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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45-702 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
GPX7, glutathione peroxidase 7 [Homo sapiens] , HGNC:4559, CL683, FLJ14777, GPX6, NPGPx, non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20kDa band observed in Human Prostate lysates (calculated MW of 21.0kDa according to NP_056511.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Background References:
  • Utomo A, Jiang X, Furuta S, Yun J, Levin DS, Wang YC, Desai KV, Green JE, Chen PL, Lee WH. Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells. J Biol Chem. 2004 Oct 15;279(42):43522-9. Epub 2004 Aug 4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CNQFGQQEPDSNK
NCBI Gene ID #:
2882
NCBI Official Name:
glutathione peroxidase 7
NCBI Official Symbol:
GPX7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056511.2
Protein GI Number:
15618997
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q96SL4