GRK2 Antibody

ProSci
Product Code: PSI-45-711
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-711-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-711 (2ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-711 (2ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
BARK1, GRK2, ADRBK1, adrenergic, beta, receptor kinase 1, BETA-ARK1, beta adrenergic receptor kinase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Daudi cell lysates (calculated MW of 79.7kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-2ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Iaccarino G, Barbato E, Cipolletta E, De Amicis V, Margulies KB, Leosco D, Trimarco B, Koch WJ. Elevated myocardial and lymphocyte GRK2 expression and activity in human heart failure. Eur Heart J. 2005 Sep;26(17):1752-8. Epub 2005 Jul 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RDAYREAQQLVQR
NCBI Gene ID #:
156
NCBI Official Name:
adrenergic, beta, receptor kinase 1
NCBI Official Symbol:
GRK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001610.2
Protein GI Number:
4501971
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P25098