hALP Antibody
| Code | Size | Price |
|---|
| PSI-45-719-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
NAT10, N-acetyltransferase 10, ALP, DKFZp434C116, FLJ10774, FLJ12179, FLJ23850, KIAA1709, hALP , N-acetyltransferase-like, N-acetyltransferase-like protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_078938.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Background References:
- Yin J, Ma Z, Selliah N, Shivers DK, Cron RQ, Finkel TH. Effective gene suppression using small interfering RNA in hard-to-transfect human T cells. J Immunol Methods. 2006 May 30;312(1-2):1-11. Epub 2006 Mar 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KSDKKRKLEAKQ
NCBI Gene ID #:
55226
NCBI Official Name:
N-acetyltransferase 10 (GCN5-related)
NCBI Official Symbol:
NAT10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078938.1
Protein GI Number:
13399322
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9H0A0