HIWI Antibody
| Code | Size | Price |
|---|
| PSI-45-735-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi, MIWI, piwi homolog, piwi-like 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6ug/ml.
Background References:
- Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26;15(2):171-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-SNRKDKYDAIKKY
NCBI Gene ID #:
9271
NCBI Official Name:
piwi-like RNA-mediated gene silencing 1
NCBI Official Symbol:
PIWIL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004755.2, NP_001177900.1
Protein GI Number:
66346725
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).
Swissprot #:
Q96J94