HMHA1 Antibody
| Code | Size | Price |
|---|
| PSI-45-738-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
HA-1, minor histocompatibility antigen HA-1, HLA-HA1, KIAA0223, HMHA1, histocompatibility (minor) HA-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65-70kDa band in Jurkat lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 131kDda according to NP_036424.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- den Haan JM, Meadows LM, Wang W, Pool J, Blokland E, Bishop TL, Reinhardus C, Shabanowitz J, Offringa R, Hunt DF, Engelhard VH, Goulmy E The minor histocompatibility antigen HA-1: a diallelic gene with a single amino acid polymorphism. Science. 1998 Feb 13;279(5353):1054-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: TLGSCRERQPEFV
NCBI Gene ID #:
23526
NCBI Official Name:
histocompatibility (minor) HA-1
NCBI Official Symbol:
HMHA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036424.2
Protein GI Number:
47834348
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)
Swissprot #:
Q92619