HNF4A Antibody
| Code | Size | Price |
|---|
| PSI-45-740-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa and 48kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1, 52.7kDa according to Mouse NP_032287.2 and 49.9kDa according to Mouse NP_001299835.1). Recommended concentration: 0.1-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2ug/ml.
Background References:
- Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR. Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish. Diabetes. 2004 Dec;53(12):3337-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: RLSKTLVDMDMADY-C
NCBI Gene ID #:
3172
NCBI Official Name:
hepatocyte nuclear factor 4, alpha
NCBI Official Symbol:
HNF4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_849180.1, NP_000448.3, NP_849181.1
Protein GI Number:
31077207
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Swissprot #:
P41235