HTATSF1 Antibody
| Code | Size | Price |
|---|
| PSI-45-752-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274(5287):605-10.; PMID: 8849451). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Zhou M, Deng L, Lacoste V, Park HU, Pumfery A, Kashanchi F, Brady JN, Kumar A. Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription. J Virol. 2004 Dec;78(24):13522-33.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QELYGDGKDGDTQTD
NCBI Gene ID #:
27336
NCBI Official Name:
HIV-1 Tat specific factor 1
NCBI Official Symbol:
HTATSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055315.2
Protein GI Number:
21361437
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O43719