IRF6 Antibody

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ProSci
Product Code: PSI-45-781
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-781-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-781 staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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45-781 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-781 staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
45-781 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Background References:
  • Kondo S, Schutte BC, Richardson RJ, Bjork BC, Knight AS, Watanabe Y, Howard E, de Lima RL, Daack-Hirsch S, Sander A, McDonald-McGinn DM, Zackai EH, Lammer EJ, Aylsworth AS, Ardinger HH, Lidral AC, Pober BR, Moreno L, Arcos-Burgos M, Valencia C, Houdayer C, Bahuau M, Moretti-Ferreira D, Richieri-Costa A, Dixon MJ, Murray JC. Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes. Nat Genet. 2002 Oct;32(2):285-9. Epub 2002 Sep 03.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TPSMQLPPALPPQ
NCBI Gene ID #:
3664
NCBI Official Name:
interferon regulatory factor 6
NCBI Official Symbol:
IRF6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006138.1
Protein GI Number:
5453700
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O14896