ITK Antibody

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ProSci
Product Code: PSI-45-784
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-784-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with 45-784 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with EB10338 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with 45-784 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with EB10338 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing full-length Human ITK (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti- DYKDDDDK tag antibody. Recommended concentration, 1-2ug/ml.
Background References:
  • Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K. A novel human tyrosine kinase gene inducible in T cells by interleukin 2. FEBS Lett 1993 Jun 7;324(1):1-5
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-RLLRQLAEIAESGL
NCBI Gene ID #:
3702
NCBI Official Name:
IL2-inducible T-cell kinase
NCBI Official Symbol:
ITK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005537.3
Protein GI Number:
15718680
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q08881