KIF4A Antibody

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ProSci
Product Code: PSI-45-798
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-798-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-798 (2ug/ml) staining of nuclear NIH3T3 (A) and (2ug/ml) negative control Human Ovary (B) lysate. (35ug protein in RIPA buffer) Detected by chemiluminescence.
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45-798 (2ug/ml) staining of nuclear NIH3T3 (A) and (2ug/ml) negative control Human Ovary (B) lysate. (35ug protein in RIPA buffer) Detected by chemiluminescence.

Documents

Further Information

Additional Names:
KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2). Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Ovary lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Background References:
  • Sekine Y, Okada Y, Noda Y, Kondo S, Aizawa H, Takemura R, Hirokawa N. A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally. J Cell Biol. 1994 Oct;127(1):187-201.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: SFFSGCSPIEEEAH
NCBI Gene ID #:
24137
NCBI Official Name:
kinesin family member 4A
NCBI Official Symbol:
KIF4A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036442.3
Protein GI Number:
7305205
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O95239