LASP1 Antibody

ProSci
Product Code: PSI-45-823
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-823-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-823 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-823 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
LASP1, LIM and SH3 protein 1, Lasp-1, MLN50
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759). Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Small Intestine. Recommended concentration: 2.5ug/ml.
Background References:
  • Grunewald TG, Kammerer U, Schulze E, Schindler D, Honig A, Zimmer M, Butt E. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res. 2006 Apr 15;312(7):974-82. Epub 2006 Jan 23. Erratum in: Exp Cell
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-PERRDSQDGSSYR
NCBI Gene ID #:
3927
NCBI Official Name:
LIM and SH3 protein 1
NCBI Official Symbol:
LASP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006139.1
Protein GI Number:
5453710
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q14847