LIMK1 Antibody

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ProSci
Product Code: PSI-79-158
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-158-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).
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Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).

Documents

Further Information

Additional Names:
LIMK, LIMK-1, C1orf215, LAP18, OP18, kinase LIMK1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
Background References:
  • Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5): 253-9.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LIMK1 (Phospho-Thr508) antibody was raised against a peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3984
NCBI Official Name:
LIM domain kinase 1
NCBI Official Symbol:
LIMK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
NP_002305.1
Protein GI Number:
4505001
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
Swissprot #:
P53667