LIMK2 Antibody

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ProSci
Product Code: PSI-79-159
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-159-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using LIMK2 (Phospho-Thr505).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (Phospho-Thr505).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK2 (Phospho-Thr505).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using LIMK2 (Phospho-Thr505).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (Phospho-Thr505).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using LIMK2 (Phospho-Thr505).

Documents

Further Information

Additional Names:
PKB, RAC, LIMK-2, kinase LIMK2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Background References:
  • Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275 (5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1 (5): 253-9.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LIMK2 (Phospho-Thr505) antibody was raised against a peptide sequence around phosphorylation site of threonine 505 (R-Y-T (p) -V-V) derived from Human LIMK2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3985
NCBI Official Name:
LIM domain kinase 2
NCBI Official Symbol:
LIMK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
NP_001026971.1
Protein GI Number:
73390140
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
Swissprot #:
P53671