LMO7 Antibody

ProSci
Product Code: PSI-45-843
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-843-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-843 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

45-843 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Further Information

Additional Names:
LMO7, FBXO20, LOMP, FBX20, KIAA0858, LIM domain only 7, LOMP protein, F-box protein Fbx20, F-box only protein 20, zinc-finger domain-containing protein, RP11-332E3.2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2ug/ml.
Background References:
  • Putilina T, Jaworski C, Gentleman S, McDonald B, Kadiri M, Wong P. Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain. Biochem Biophys Res Commun. 1998 Nov 18;252(2):433-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: ESTCVSASLPRS
NCBI Gene ID #:
4008
NCBI Official Name:
LIM domain 7
NCBI Official Symbol:
LMO7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAD33924.1
Protein GI Number:
4929268
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Please note that the amino acid sequence of the immuning peptide differs one amino acid from the current NCBI accession numbers (NP_005349.3, NP_056667.2). This antibody is expected to recognise both reported isoforms (NP_005349.3; NP_056667.2).
Swissprot #:
Q8WWI1