MRG15 Antibody

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ProSci
Product Code: PSI-45-901
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-901-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-901 (0.3ug/ml) staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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45-901 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-901 (0.3ug/ml) staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-901 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>

Documents

Further Information

Additional Names:
MORF4L1, MRG15, MORF-related gene 15, mortality factor 4 like 1, Eaf3, FWP006, HsT17725, MEAF3, MGC10631, MORFRG15, Esa1p-associated factor 3 homolog, MORF-related gene on chromosome 15
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in nuclear lysates of cell lines HepG2 and NIH3T3 and approx 40kDa band observed in Mouse, Rat and Pig Liver lysates (calculated MW of 37.2kDa according to Human NP_006782.1, Mouse NP_077751.1, Rat NP_001011999.1 and Pig XP_001927776.1 and 41.5kDa according to Human NP_996670.1, Mouse NP_001034236.1 and Pig XP_001927789.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Pardo PS, Leung JK, Lucchesi JC, Pereira-Smith OM. MRG15, a Novel Chromodomain Protein, Is Present in Two Distinct Multiprotein Complexes Involved in Transcriptional Activation. J Biol Chem. 2002 Dec 27;277(52):50860-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: APKQDPKPKFQEG-C
NCBI Gene ID #:
10933
NCBI Official Name:
mortality factor 4 like 1
NCBI Official Symbol:
MORF4L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006782.1, NP_996670.1
Protein GI Number:
5803102
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).
Swissprot #:
Q9UBU8