MRIP1 Antibody

ProSci
Product Code: PSI-45-902
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-902-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-902 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-902 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
AGAP3, CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, CRAG, FLJ16146, FLJ34452, CRMP (collapsin response mediator protein) associated, centaurin, gamma 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.
Background References:
  • Surks HK, Richards CT, Mendelsohn ME. Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. J Biol Chem. 2003 Dec 19;278(51):51484-93. Epub 2003 Sep 23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-NPSAELHRSPSLL
NCBI Gene ID #:
116988
NCBI Official Name:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
NCBI Official Symbol:
AGAP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_114152.3
Protein GI Number:
110227613
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q96P47