MYF5 Antibody

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ProSci
Product Code: PSI-45-926
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-926-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-926 (0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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45-926 (0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MYF5 , myogenic factor 5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Background References:
  • Kuang S, Kuroda K, Le Grand F, Rudnicki MA. Asymmetric self-renewal and commitment of satellite stem s in muscle. . 2007 Jun 1;129(5):999-1010.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PECNSPVWSRKSST
NCBI Gene ID #:
4617
NCBI Official Name:
myogenic factor 5
NCBI Official Symbol:
MYF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005584.1
Protein GI Number:
5031929
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P13349