NANOS1 Antibody

ProSci
Product Code: PSI-45-933
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-933-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-933 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-933 (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
NANOS1, nanos homolog 1 (Drosophila), NOS1, nanos homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13(7):727-39. Epub 2004 Feb 12, PMID: 14962983). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Sajek et al (2018) PMID: 30269240.
Background References:
  • Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev Genes Evol. 2003 Apr;213(3):120-6. Epub 2003 Feb 11.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SARDGPPGKKLR
NCBI Gene ID #:
340719
NCBI Official Name:
nanos homolog 1 (Drosophila)
NCBI Official Symbol:
NANOS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_955631.1
Protein GI Number:
40804761
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q8WY41