Neuroligin 1 Antibody

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ProSci
Product Code: PSI-45-957
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-957-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-957 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i
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45-957 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i

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Further Information

Additional Names:
NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10ug/ml.
Background References:
  • Chih B, Engelman H, Scheiffele P. Control of Excitatory and Inhibitory Synapse Formation by Neuroligins. Science. 2005 Jan 27;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-TAKQDDPKQQ
NCBI Gene ID #:
22871
NCBI Official Name:
neuroligin 1
NCBI Official Symbol:
NLGN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055747.1
Protein GI Number:
7662470
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q8N2Q7