Neurturin Antibody

ProSci
Product Code: PSI-45-963
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-963-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-963 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-963 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
Nrtn, neurturin, NTN
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 22-23kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 22.4kDa according to mouse NP_032764.1). Recommended concentration: 0.1-0.3ug/ml. An additional very strong band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Ye M, Wang XJ, Zhang YH, Lu GQ, Liang L, Xu JY, Chen SD. Transplantation of bone marrow stromal cells containing the neurturin gene in rat model of Parkinson's disease. Brain Res. 2007 Apr 20;1142:206-16. Epub 2006 Dec 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DVHSRYHTLQELSARE
NCBI Gene ID #:
18188 (mouse); 84423 (rat);
NCBI Official Name:
neurturin
NCBI Official Symbol:
Nrtn
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_032764.1
Protein GI Number:
6679136
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P97463