NFATC4 Antibody

ProSci
Product Code: PSI-45-964
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-964-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-964 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-964 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
NFATC4, NFAT3, NF-ATc4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, T cell transcription factor NFAT3, nuclear factor of activated T-cells, cytoplasmic 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:No signal obtained yet but low background observed in MOLT-4, Jurkat, A431, HeLa, Human Brain and Human Heart lysates at upto 2ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows weak nuclear staining in trophoblasts.. Recommended concentration, 2.5-3.8ug/ml.
Background References:
  • Hoey T, Sun YL, Williamson K, Xu X. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. Immunity 1995 May;2(5):461-72
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-SGFPAPPGEEPPA
NCBI Gene ID #:
4776
NCBI Official Name:
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
NCBI Official Symbol:
NFATC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004545.2, NP_001185894.1, NP_001185895.1, NP_001185896.1
Protein GI Number:
27886563
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 2, 3, 4 and 5 (NP_004545.2; NP_001185894.1; NP_001185895.1; NP_001185896.1 respectively).
Swissprot #:
Q14934