NTE Antibody
| Code | Size | Price |
|---|
| PSI-42-227-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PNPLA6, patatin-like phospholipase domain containing 6, NTE, NTEMND, SPG39, sws, neuropathy target esterase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 75kDa band in lysates of cell lines HeLa and Kelly after 2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 146kDa according to NP_006693.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Rainier S, Bui M, Mark E, Thomas D, Tokarz D, Ming L, Delaney C, Richardson RJ, Albers JW, Matsunami N, Stevens J, Coon H, Leppert M, Fink JK. Neuropathy target esterase gene mutations cause motor neuron disease. Am J Hum Genet. 2008 Mar;82(3):780-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse
Immunogen:
The immunogen for this antibody is: C-YQGRQTSSIFEAAKQ
NCBI Gene ID #:
10908
NCBI Official Name:
patatin-like phospholipase domain containing 6
NCBI Official Symbol:
PNPLA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006693.3
Protein GI Number:
116256487
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
Swissprot #:
Q8IY17