Connexin Antibody

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ProSci
Product Code: PSI-50-200
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-200-0.1mL0.1mL£686.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Avian
  • Canine (dog)
  • Fish
  • Human
  • Mouse
  • Rat
  • Sheep
Application: Western Blot (WB)
Storage:
Connexin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.
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Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.

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Further Information

Application Note:
The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Background References:
  • Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
  • Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
  • Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
NCBI Gene ID #:
24392
NCBI Official Name:
gap junction protein, alpha 1
NCBI Official Symbol:
Gja1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43
Protein Accession #:
P08050
Protein GI Number:
117708
Purification:
Affinity Purified
Swissprot #:
P08050
User NOte:
Optimal dilutions for each application to be determined by the researcher.