IL-11 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5164-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-11 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
AGIF, IL-11Interleukin-11, Adipogenesis inhibitory factor, IL-11
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-11 (1.50 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required.
ELISA:
To detect hIL-11 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-11.
Sandwich:
To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-11 (XP-5164Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.
Western Blot:
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-11 (1.50 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required.
ELISA:
To detect hIL-11 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-11.
Sandwich:
To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-11 (XP-5164Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.
Western Blot:
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-11 (human Interleukin-11).
NCBI Gene ID #:
3589
NCBI Official Name:
interleukin 11
NCBI Official Symbol:
IL11
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P20809
Protein GI Number:
124294
Purification:
Anti-hIL-11 specific antibody was purified by affinity chromatography employing immobilized hIL-11 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Neuroscience,Antibody Pairs, Functional Assays
Swissprot #:
P20809
User NOte:
Centrifuge vial prior to opening.