IL-19 Antibody

ProSci
Product Code: PSI-38-132
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-132-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IL-19 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hIL-19 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-19 (38-291) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-19.
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To detect hIL-19 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-19 (38-291) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-19.

Further Information

Additional Names:
MDA1, NG.1, ZMDA1, IL-10C, Interleukin-19, Melanoma differentiation-associated protein-like protein, IL-19
Application Note:
ELISA:

Sandwich:
To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-19 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-19.

Western Blot:
To detect hIL-19 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography and then biotinylated.ced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography employing immobilized hIL-19 matrix.
NCBI Gene ID #:
29949
NCBI Official Name:
interleukin 19
NCBI Official Symbol:
IL19
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9UHD0
Protein GI Number:
146345440
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
Q9UHD0
User NOte:
Centrifuge vial prior to opening.